2nd European MicroCal meeting – Institut Pasteur, Paris, Sept. 26-27, 2016
This meeting, co-organized by ARBRE-MOBIEU and Malvern Instruments with the support of Institut Pasteur, brought together 120 scientists from 20 countries. It allowed this wide user community to share and broaden their experience, and to network on topics related with applications, best practices, advanced data analysis and latest developments in isothermal titration calorimetry and differential scanning calorimetry (ITC and DSC).
Principal organisers: Patrick England (Institut Pasteur and ARBRE-MOBIEU) & Natalia Markova (Malvern Instruments).
Meeting photo – Institut Pasteur
Meeting poster and brochure/abstract book. To see each presentation, please click on the speaker’s name.
| Agenda Monday, September 26th (Amphithéâtre Duclaux) |
| Meeting Opens | |
| 08:00-08:45 | Registration and poster installation |
| 08:45-09:00 | Opening remarks by the host and chair of ARBRE MOBIEU, Patrick England, Institut Pasteur, Paris, France |
| Morning Session 1 Interaction analysis: The value of orthogonal approaches Chair: Patrick England, Institut Pasteur, Paris, France |
|
| 09:00-09:30 | “The Power of Orthogonal Biophysical Methods Exemplified by An Integrative Structural Biology Study of FGFR1″
Geoff Holdgate, AstraZeneca, Cambridge, UK |
| 09:30-09:50 | “Combining isothermal titration calorimetry, fluorescence anisotropy and kinetic approaches to decipher the molecular mechanisms of the redox activation of the Yap1 transcription factor in S. cerevisiae”
Sophie Rahuel-Clermont, Université de Lorraine, Nancy, France |
| 09:50-10:10 | “What have we learned from the enthalpy database of 200 compound binding to 8 carbonic anhydrase isoforms as determined by isothermal titration calorimetry”
Daumantas Matulis, Vilnius University, Lithuania |
| 10:10-10:30 | Flash presentation of posters (1 slide & 2min/talk) 9 speakers :
Regina Adao, University of Campinas, Sao Paulo, Brazil |
| 10:30-11:00 | Coffee break and 1st poster session |
| Morning Session 2 Focus on DSC: Differential Scanning Calorimetry Chair: Michaela Wimmerova, Brno, Czech Republic |
|
| 11:00-11:30 | “Thermal dissociation and unfolding of insulin and effects of ligand interactions studied by DSC. Predictability of insulin analogue stability using DSC and correlation with other methods”
Kasper Huus, Novo Nordisk, Denmark |
| 11:30-11:50 | “Study of rabies virus by Differential Scanning Calorimetry: Identification of Proteins Involved in Thermal Transitions”
Frederic Greco, Sanofi Pasteur, Marcy l’Etoile, France |
| 11:50-12:10 | “Using Differential Scanning Calorimetry to monitor glioblastoma evolution”
François Devred, Université Aix-Marseille, France |
| 12:10-12:30 | “DSC in application to the studies of blood serum samples”
Adrian Velazquez Campoy, Zaragoza University, Spain |
| 12:30-13:30 | Lunch |
| 13:30-14:15 | Panel discussion 1
“Best practices in DSC experimental setup and data analysis” |
| Afternoon session 1 Focus on ITC: Isothermal Titration Calorimetry Chair: Chris Johnson, Cambridge, UK |
|
| 14:15-14:35 | “Study of peptide/lipid interactions by ITC and DSC”
Isabel Alves, CBMN, Bordeaux, France |
| 14:35-14:55 | “Enthalpic Forces Correlate with the Selectivity of Transthyretin-Stabilizing Ligands in Human Plasma”
Kristoffer Brännström, Umea University, Sweden |
| 14.55-15:15 | “Affinity based definition of RNA motifs and strategic protein-RNA mutational design”
Cameron Mackereth, IECB, Bordeaux, France |
| 15:15-15:45 | Coffee break and 2nd poster session |
| Afternoon session 2 Novel developments in the field of calorimetry Chair: Margarida Bastos, Porto, Portugal |
|
| 15:45-16:05 | “The underestimated potential of isothermal microcalorimetry for microbiological studies”
Olivier Braissant, Basel University, Switzerland |
| 16:05:16:25 | “ITC studies of enzyme activity in solution and suspensions”
Peter Westh, Roskilde University, Denmark |
| 16:25-16:45 | “Use of isothermal titration calorimetry to investigate and identify novel peptide substrates for prolyl carboxypeptidase. A technology comparison”
Anne-Marie Lambeir, Antwerp University, Belgium |
| 16:45-17:05 | KinITC – Kinetic Revolution in Drug Discovery”
Pascal Zihlmann, Basel University, Switzerland |
| 17:05-17:50 | Panel discussion 2 “Challenges and best practices for the application of ITC to the studies of enzyme kinetics” Chaired by Natalia Markova, Malvern Instruments, UK |
| 17:50-19:30 | 3rd poster session |
| 19:30-21:15 | Gala Dinner |
| Agenda Tuesday, September 27th (Amphithéâtre Duclaux) |
| Morning Session 1 Developments in ITC applications into challenging areas Chair: Aymeric Audfray, Malvern Instruments Lyon, France |
|
| 09:00-09:30 | “ITC for Structural Glycosciences tools”
Anne Imberty, CERMAV-CNRS, Grenoble, France |
| 09:30-09:50 | “ITC as a tool for the characterization of self-assembled systems in organic solvents”
Laurent Bouteiller, Pierre and Marie Curie University, Paris, France |
| 09:50-10:30 | Panel discussion 3 “Challenges and best practices for the use of ITC in non-traditional applications“Chaired by Aymeric Audfray, Malvern Instruments Lyon, France |
| 10:30-11:00 | Coffee break and 4th poster session |
| Morning Session 2 Binding complexity and data quality with ITC Chair: Adrian Velazquez-Campoy, Zaragoza, Spain |
|
| 11:00-11:30 | “Any Kd you like”
Joe Coyle, Astex Pharmaceuticals, Cambridge, UK |
| 11:30-11:50 | “Improved characterization of supramolecular assemblies by non conventional Isothermal Titration Calorimetry experiments”
David Landy, Université du Littoral, Dunkerque, France |
| 11:50-12:10 | “10 good reasons to perform ITC experiments”
Eric Ennifar, Strasbourg University, France |
| 12:10-12:55 | Panel discussion 4 “Best practices in ITC data analysis : quality optimization, and appraisal of complex binding and kinetics” Chaired by Eva Munoz, AFFINImeter, Santiago de Compostela, Spain, and Adrian Velazquez-Campoy, Zaragoza University, Spain |
| 12:55-13:15 | Concluding remarks Patrick England , Institut Pasteur, Paris, France and Natalia Markova, Malvern Instruments, UK |
| 13:15-14:00 | Lunch |