Thomas Jowitt, University of Manchester, UK together with several members of the WG set up a survey on the general need of new standard systems. The survey has been filled in by members of the ARBRE/MOBIEU community and analysis was performed by Thom Jowitt. A key result is that there is indeed an unmet need for standard samples, especially for interaction studies.
Development of a new standard system
There were several attempts to develop new standard systems for the biophysical characterization of interactions. A very promising system has been established in Thomas Jowitt’s lab, University of Manchester, UK: the binding of lysozyme to a nanobody the affinity of which can be tued by targeted amino acid exchange. After comprehensive characterization it has been used as model system in the MST benchmarking study. A general overview of this system has been presented by Thomas Jowitt at a WG meeting in 2017 New_standards_lysozyme_nanobody.PDF