Technological approaches

  • Hydrodynamic approaches:
    analytical ultracentrifugation (AUC), light scattering (dynamic and static), small-angle X-ray and neutron scattering (SAXS-SANS), microscale thermophoresis (MST), electrophoretic mobility, precision viscometry, ...
  • Spectroscopies:
    fluorescence, infrared, circular dichroism (CD), Raman, electron spin resonance (ESR), nuclear magnetic resonance (NMR; for aims other than structure determination, e.g. the study of conformational dynamics and interactions), ...
  • Real-time biosensing:
    based on: surface plasmon resonance (SPR), quartz crystal microbalance (QCM), plasmon waveguide resonance (PWR), interferometry (biolayer (BLI), dual polarization (DPI) or backscattering (BSI)), ...
  • Fast and ultra-fast kinetics:
    using for instance stopped-flow and temperature jump devices
  • Microcalorimetry:
    isothermal (ITC) and differential scanning (DSC), differential scanning fluorimetry (DSF)
  • Single molecule approaches:
    atomic force microscopy (AFM), single-molecule FRET (smFRET), optical tweezers
  • Structural mass spectrometry:
    ion mobility MS (IM-MS), chemical cross-linking coupled with MS (CX-MS),hydrogen-deuterium exchange MS (HDX-MS)
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