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Meeting Programme

Molecular Biophysics – ABC of the puzzle of Life

ARBRE-MOBIEU Plenary Meeting. Zagreb, March 18-20, 2019.

Venue: Hotel Panorama, Trg Krešimira Ćosića 9, Zagreb, Croatia

March 18, 2019 (Monday)

10:00 – 12:30 Core group meeting (WG leaders/co-leaders only) &
arrival (all )
12:00 – 13:00Registration
12:30 – 13:30 Lunch
13:30 – 14:00 Welcome and Introduction
Session A - Assemblies and Interactions of Bio(macro)molecules
Chair: Tomislav Vuletić
14:00  – 14:35 Keynote lecture: Protein synthesis: from ribosome assembly to targeting of membrane proteins. Nenad Ban. Switzerland.
Selected Abstracts:
14:35 – 14:55 Unexpected magnitudes of SPR signals explained by a new model. Jérôme Dejeu, France.
14:55 – 15:15Biophysics of Viral Assembly: Studies at the Single-Virus Level. Wouter H. Roos, The Netherland.
15:15 – 15:35Unravelling the Oligomerization State of the T Cell Antigen Receptor. Mario Brameshuber, Austria.
Sponsor presentation:
15:35 – 15:45EPR: Structural Insight and Sample Quality. Patrick Carl (Bruker Biospin)
15:45 – 16:10 Coffee Break
Chair: Krzysztof Skowronek
16:10 – 16:20 Characterizing conformational changes by second-harmonic generation (SHG) A novel technology for monitoring biomolecule on-target binding and differentiating mechanism of action. Bruce Mortensen (Biodesy).
Selected Abstracts:
16:20 - 16:40Interactions of the disordered oncoprotein Myc - biophysics paves way for understanding and interruption of tumor formation. Maria Sunnerhagen, Sweden.
16:40 - 17:00Structure-functional study of lectins: key players in a fight between host and pathogen. Josef Houser, Czech Republic.
17:00 - 17:20Label-free characterization of cell-surface glycans-lectins interactions as test for melanoma development. Tomasz Kobiela, Poland.
17:20 - 17:40Fluorescence anisotropy based assay for monitoring of interactions between ligand and protein. Ago Rinken, Estonia.
17:40 - 18:00Flash Poster Presentations Session 1
Chair: Isabel D. Alves
1. Exploring the dynamic structure of bionanocages. João Jacinto, Portugal.
2. Long, highly-stable and flexible: what analytical ultracentrifugation can tell us about “random walk” oligomer. Alexandra Solovyova, United Kingdom.
3. ITC: theory, practice and pitfalls. Philippe Dumas, France.
4. Amyloid β-Protein Inhibition and Interaction with Membranes. Rita Carrotta, Italy.
5. Study of The Human Dipeptidyl Peptidase III Interactom.Sanja Tomic, Croatia.
6. Heparanase binds substrate via a dynamic binding mechanism. Aleksandra Marsavelski, Croatia.
18:00 – 20:00 Poster Session 1

March 19, 2019 (Tuesday)

Session B - Bio-Interfaces, Biomaterials and Membranes
Chair: Margarida Bastos
Selected Abstracts:
9:00 - 9:20 From ARBRE-MOBIEU networking to regional research cooperation: Multimethod study of algal cell response to laboratory-induced cadmium stress. . Nadica Ivošević DeNardis, Croatia.
9:20 - 9:40Nanoscale Mechanosensing of Natural Killer Cells is Revealed by Antigen-Functionalized Nanowires. Mark Schvartzmann, Israel.
9:40 - 10:00 Deciphering putative lipid membrane interaction sites of the TRAP1 channel by Microfluidic Diffusional Sizing.Isabel D. Alves, France.
10:00 - 10:20 Efficient antimicrobial peptides against multiresistant bacteria presenting also anticancer activity. Nuno Santos, Portugal.
Sponsor presentations:
10:20 - 10:30 What happens in solution should stay in solution: Recent needs and how centrifugation can help with preparation and characterisation of proteins and "biological structures". Lutz Ehrhardt (Beckman Coulter).
10:30 - 10:40 The switchSENSE technology as a versatile tool for elucidating the mechanism of action of small molecule drug candidates. Lena Kilian (Dynamic Biosensors).
10:40  –  11:10 Coffee break
Chair: Tina Daviter
Sponsor presentations:
11:10 - 11:20 Measurement of stoichiometry of protein and membrane protein complexes by light scattering coupled to SEC. Christoph Albermann (Wyatt).
11:20 - 11:30 Application of Temperature Related Intensity Change (TRIC) in biophysical drug discovery projects. Matthias Molnar (NanoTemper).
Selected Abstracts:
11:30 - 11:50Interaction Between Tethered Bilayer Membranes and s100A9 aggregates. Rima Budvytyte, Lithuania.
11:50-12:20 Flash Poster Presentations Session 2:
1. Bead Injection Multiplex Immunoanalysis. Toonika Rinken, Estonia.
2. EIS and SERS Analysis of Anchor Molecules for Tethered Bilayer Lipid Membrane Formation. Indre Aleknaviciene, Lithuania.
3. Algal cells under heavy metal stress: physiological and morphological response. Tea Mišić Radić, Croatia.
4. Quaternary Structure of Human NK Cell Receptor:Ligand complexes by the Looking Glass of Super-resolution Microscopy. Barbora Kalousková, Czech Republic.
5. Differential Scanning Fluorimetry: revisiting microtubules assembly. François Devred, France.
6. Structural and functional evidence for plasticity of Potato virus Y coat protein. Marjetka Podobnik, Slovenia.
7. Ferritin: The most interesting bionano component? Soumyananda Chakraborti, Poland.
8. Probing the mechanism of iron release from Dps, a miniferritn. Ana V Almeida, Portugal.
9. Plant seryl-tRNA synthetase as a link between translation and metabolism of brassinosteroid hormones. Jasmina Rokov-Plavec, Croatia.
12:20 - 13:40 Lunch & poster session 2
Session C - Controling Sample Quality, Instruments and Standards
Chair: Bertrand Raynal
13:40 – 14:15 Keynote lecture Optimized sample preparation for cryo-electron tomography of cellular lamellasJiří Nováček Jiri Novacek. Czech Republic.
   14:05 – 15:05 Selected Abstracts :
14:15  –  14:35 An improved facility for rapid-mixing multi-angle light scattering (MALS) coupled to small-angle X-ray scattering (SAXS) at the Synchrotron SOLEIL SWING beamline. Mattia Rocco, Italy.
14:35  –  14:55ITC benchmark study: test reactions and data analysis - a case study within ARBRE-MOBIEU COST action. Margarida Bastos, Portugal.
15:05 - 15:35 Sponsor presentations:
14:55 - 15:05 Microfluidic Diffusional Sizing (MDS) for protein characterization – latest results and next step. Jonathan Faherty (Fluidic Analytics Ltd)
15:05nbsp;- 15:15 Beyond α-helix and β-sheet: Expanding the Role of Circular Dichroism. Martin Textor (Applied Photophysics Inc).
15:15  –  15:45Coffee break
Chair: Adriana E. Miele
Sponsor presentation:
15:45  –  15:55Flow Induced Dispersion Analysis (FIDA) quantifies proteins, protein-ligand interactions and protein stability under native conditions. Henrik Jensen (FIDA-Tech Aps).
Selected Abstracts :
15:55 –  16:15 Kinetic stability of proteins - How stable is stable? Didier Clénet, France.
16:15 –  16:35 Monitoring of a DNA double helix formation: a multi-approach benchmark study. Carmelo Di Primo, France.
Sponsor presentation:
16:35 –  16:45 Integrated Solutions for Early Stage Biologics Discovery. Hendrik Wünsche (Pall ForteBio).
16:45-17:20 Flash Poster Presentations 3
Chair: Ondřej Vaněk
1. Development of a SANS strategy for the study of membrane proteins: application to a prokaryotic NADPH oxidase homolog. Christine Ebel, France.
2. Alzheimer’s disease from a single molecule/single cell perspective. Martino Calamai, Italy.
3. Cell Adhesion and Mechanics in Fibroblasts and Myofibroblasts. Manfred Radmacher, Germany.
Studies
4. Controlling enzymatic activity by immobilization. Marek Wiśniewski, Poland.
5. Tethered Bilayer Lipid Membranes – a Comprehensive Tool for electrochemical Studies of Pore-Forming Toxins. Tadas Penkauskas, Lithuania.
6. Protein-protein interaction standards: The latest news in attempts to make ideal nanobodies. Tom Jowitt, United Kingdom.
7. Development of an Infrared Spectroscopy Data Repository. Tina Daviter, United Kingdom.
8. Macromolecular interactions in vitro, comparing classical and new approaches. Paloma Fernandez-Varela, France.
9. Precision of ITC measurements for target-based drug design. Daumantas Matulis, Lithuania.
17:20 - 19:30 Poster Session 3
18:00 - 19:30 Full Management Committee Meeting (members only).
20:00Social Dinner

March 20, 2019 (Wednesday)

COST Actions Session.
Chair: Patrick England
9:00 – 9:35 Keynote lecture: Structural investigations of striated muscle Z-disks: fuzzy α-Actinin interactome & Presentation of COST Action “NGPnet” (Non-Globular Proteins) Kristina Djinovic-Carugo. Austria.
9:35 - 9:55 Presentation of COST Action “BioBrillouin”. Kareem Elsayad, Austria.
9:55 - 10:15 Presentation of COST Action “Native Mass Spectrometry”. Frank Sobott, United Kingdom.
10:15 - 10:35
Presentation of COST Action “Molecular Machines”. Fraser MacMillan, United Kingdom.
10:35 - 11:00 Coffee break
11:00 – 12:30 ARBRE-MOBIEU General Assembly: Summary of Achievements during the Third Year of the Action and future perspectives.
12:30 Announcement of best poster & best talk prize & Concluding Remarks
13:00Departure

COST Action CA15126

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Biomedicine and Molecular Biosciences Action CA15126 is funded by COST, which is supported by the EU Framework Programme Horizon 2020.

COST Action CA15126

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