ARBRE:
Association of Resources for Biophysical Research in Europe
MOBIEU:
Molecular Biophysics in Europe
Meeting Programme
Molecular Biophysics – ABC of the puzzle of Life
ARBRE-MOBIEU Plenary Meeting. Zagreb, March 18-20, 2019.
Venue: Hotel Panorama, Trg Krešimira Ćosića 9, Zagreb, Croatia
March 18, 2019 (Monday)
| 10:00 – 12:30 | Core group meeting (WG leaders/co-leaders only) & arrival (all ) |
| 12:00 – 13:00 | Registration |
| 12:30 – 13:30 | Lunch |
| 13:30 – 14:00 | Welcome and Introduction |
| Session A - Assemblies and Interactions of Bio(macro)molecules | |
| Chair: Tomislav Vuletić | |
| 14:00 – 14:35 | Keynote lecture: Protein synthesis: from ribosome assembly to targeting of membrane proteins. Nenad Ban. Switzerland. |
| Selected Abstracts: | |
| 14:35 – 14:55 | Unexpected magnitudes of SPR signals explained by a new model. Jérôme Dejeu, France. |
| 14:55 – 15:15 | Biophysics of Viral Assembly: Studies at the Single-Virus Level. Wouter H. Roos, The Netherland. |
| 15:15 – 15:35 | Unravelling the Oligomerization State of the T Cell Antigen Receptor. Mario Brameshuber, Austria. |
| Sponsor presentation: |
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| 15:35 – 15:45 | EPR: Structural Insight and Sample Quality. Patrick Carl (Bruker Biospin) |
| 15:45 – 16:10 | Coffee Break |
| Chair: Krzysztof Skowronek | |
| 16:10 – 16:20 | Characterizing conformational changes by second-harmonic generation (SHG) A novel technology for monitoring biomolecule on-target binding and differentiating mechanism of action. Bruce Mortensen (Biodesy). |
| Selected Abstracts: | |
| 16:20 - 16:40 | Interactions of the disordered oncoprotein Myc - biophysics paves way for understanding and interruption of tumor formation. Maria Sunnerhagen, Sweden. |
| 16:40 - 17:00 | Structure-functional study of lectins: key players in a fight between host and pathogen. Josef Houser, Czech Republic. |
| 17:00 - 17:20 | Label-free characterization of cell-surface glycans-lectins interactions as test for melanoma development. Tomasz Kobiela, Poland. |
| 17:20 - 17:40 | Fluorescence anisotropy based assay for monitoring of interactions between ligand and protein. Ago Rinken, Estonia. |
| 17:40 - 18:00 | Flash Poster Presentations Session 1 |
| Chair: Isabel D. Alves | |
| 1. Exploring the dynamic structure of bionanocages. João Jacinto, Portugal. | |
| 2. Long, highly-stable and flexible: what analytical ultracentrifugation can tell us about “random walk” oligomer. Alexandra Solovyova, United Kingdom. | |
| 3. ITC: theory, practice and pitfalls. Philippe Dumas, France. | |
| 4. Amyloid β-Protein Inhibition and Interaction with Membranes. Rita Carrotta, Italy. | |
| 5. Study of The Human Dipeptidyl Peptidase III Interactom.Sanja Tomic, Croatia. | |
| 6. Heparanase binds substrate via a dynamic binding mechanism. Aleksandra Marsavelski, Croatia. | |
| 18:00 – 20:00 | Poster Session 1 |
March 19, 2019 (Tuesday)
| Session B - Bio-Interfaces, Biomaterials and Membranes |
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| Chair: Margarida Bastos | |
| Selected Abstracts: | |
| 9:00 - 9:20 | From ARBRE-MOBIEU networking to regional research cooperation: Multimethod study of algal cell response to laboratory-induced cadmium stress. . Nadica Ivošević DeNardis, Croatia. |
| 9:20 - 9:40 | Nanoscale Mechanosensing of Natural Killer Cells is Revealed by Antigen-Functionalized Nanowires. Mark Schvartzmann, Israel. |
| 9:40 - 10:00 | Deciphering putative lipid membrane interaction sites of the TRAP1 channel by Microfluidic Diffusional Sizing.Isabel D. Alves, France. |
| 10:00 - 10:20 | Efficient antimicrobial peptides against multiresistant bacteria presenting also anticancer activity. Nuno Santos, Portugal. |
| Sponsor presentations: | |
| 10:20 - 10:30 | What happens in solution should stay in solution: Recent needs and how centrifugation can help with preparation and characterisation of proteins and "biological structures". Lutz Ehrhardt (Beckman Coulter). |
| 10:30 - 10:40 | The switchSENSE technology as a versatile tool for elucidating the mechanism of action of small molecule drug candidates. Lena Kilian (Dynamic Biosensors). |
| 10:40 – 11:10 | Coffee break |
| Chair: Tina Daviter | |
| Sponsor presentations: | |
| 11:10 - 11:20 | Measurement of stoichiometry of protein and membrane protein complexes by light scattering coupled to SEC. Christoph Albermann (Wyatt). |
| 11:20 - 11:30 | Application of Temperature Related Intensity Change (TRIC) in biophysical drug discovery projects. Matthias Molnar (NanoTemper). |
| Selected Abstracts: | |
| 11:30 - 11:50 | Interaction Between Tethered Bilayer Membranes and s100A9 aggregates. Rima Budvytyte, Lithuania. |
| 11:50-12:20 | Flash Poster Presentations Session 2: |
| 1. Bead Injection Multiplex Immunoanalysis. Toonika Rinken, Estonia. | |
| 2. EIS and SERS Analysis of Anchor Molecules for Tethered Bilayer Lipid Membrane Formation. Indre Aleknaviciene, Lithuania. | |
| 3. Algal cells under heavy metal stress: physiological and morphological response. Tea Mišić Radić, Croatia. | |
| 4. Quaternary Structure of Human NK Cell Receptor:Ligand complexes by the Looking Glass of Super-resolution Microscopy. Barbora Kalousková, Czech Republic. | |
| 5. Differential Scanning Fluorimetry: revisiting microtubules assembly. François Devred, France. | |
| 6. Structural and functional evidence for plasticity of Potato virus Y coat protein. Marjetka Podobnik, Slovenia. | |
| 7. Ferritin: The most interesting bionano component? Soumyananda Chakraborti, Poland. | |
| 8. Probing the mechanism of iron release from Dps, a miniferritn. Ana V Almeida, Portugal. | |
| 9. Plant seryl-tRNA synthetase as a link between translation and metabolism of brassinosteroid hormones. Jasmina Rokov-Plavec, Croatia. | |
| 12:20 - 13:40 | Lunch & poster session 2 |
| Session C - Controling Sample Quality, Instruments and Standards | |
| Chair: Bertrand Raynal | |
| 13:40 – 14:15 | Keynote lecture Optimized sample preparation for cryo-electron tomography of cellular lamellasJiří Nováček Jiri Novacek. Czech Republic. |
| 14:05 – 15:05 | Selected Abstracts : |
| 14:15 – 14:35 | An improved facility for rapid-mixing multi-angle light scattering (MALS) coupled to small-angle X-ray scattering (SAXS) at the Synchrotron SOLEIL SWING beamline. Mattia Rocco, Italy. |
| 14:35 – 14:55 | ITC benchmark study: test reactions and data analysis - a case study within ARBRE-MOBIEU COST action. Margarida Bastos, Portugal. |
| 15:05 - 15:35 | Sponsor presentations: |
| 14:55 - 15:05 | Microfluidic Diffusional Sizing (MDS) for protein characterization – latest results and next step. Jonathan Faherty (Fluidic Analytics Ltd) |
| 15:05nbsp;- 15:15 | Beyond α-helix and β-sheet: Expanding the Role of Circular Dichroism. Martin Textor (Applied Photophysics Inc). |
| 15:15 – 15:45 | Coffee break |
| Chair: Adriana E. Miele | |
| Sponsor presentation: | |
| 15:45 – 15:55 | Flow Induced Dispersion Analysis (FIDA) quantifies proteins, protein-ligand interactions and protein stability under native conditions. Henrik Jensen (FIDA-Tech Aps). |
| Selected Abstracts : | |
| 15:55 – 16:15 | Kinetic stability of proteins - How stable is stable? Didier Clénet, France. |
| 16:15 – 16:35 | Monitoring of a DNA double helix formation: a multi-approach benchmark study. Carmelo Di Primo, France. |
| Sponsor presentation: | |
| 16:35 – 16:45 | Integrated Solutions for Early Stage Biologics Discovery. Hendrik Wünsche (Pall ForteBio). |
| 16:45-17:20 | Flash Poster Presentations 3 |
| Chair: Ondřej Vaněk | |
| 1. Development of a SANS strategy for the study of membrane proteins: application to a prokaryotic NADPH oxidase homolog. Christine Ebel, France. | |
| 2. Alzheimer’s disease from a single molecule/single cell perspective. Martino Calamai, Italy. | |
| 3. Cell Adhesion and Mechanics in Fibroblasts and Myofibroblasts. Manfred Radmacher, Germany. Studies |
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| 4. Controlling enzymatic activity by immobilization. Marek Wiśniewski, Poland. | |
| 5. Tethered Bilayer Lipid Membranes – a Comprehensive Tool for electrochemical Studies of Pore-Forming Toxins. Tadas Penkauskas, Lithuania. | |
| 6. Protein-protein interaction standards: The latest news in attempts to make ideal nanobodies. Tom Jowitt, United Kingdom. | |
| 7. Development of an Infrared Spectroscopy Data Repository. Tina Daviter, United Kingdom. | |
| 8. Macromolecular interactions in vitro, comparing classical and new approaches. Paloma Fernandez-Varela, France. | |
| 9. Precision of ITC measurements for target-based drug design. Daumantas Matulis, Lithuania. | |
| 17:20 - 19:30 | Poster Session 3 |
| 18:00 - 19:30 | Full Management Committee Meeting (members only). |
| 20:00 | Social Dinner |
March 20, 2019 (Wednesday)
| COST Actions Session. | |
| Chair: Patrick England | |
| 9:00 – 9:35 | Keynote lecture: Structural investigations of striated muscle Z-disks: fuzzy α-Actinin interactome & Presentation of COST Action “NGPnet” (Non-Globular Proteins) Kristina Djinovic-Carugo. Austria. |
| 9:35 - 9:55 | Presentation of COST Action “BioBrillouin”. Kareem Elsayad, Austria. |
| 9:55 - 10:15 | Presentation of COST Action “Native Mass Spectrometry”. Frank Sobott, United Kingdom. |
| 10:15 - 10:35 | Presentation of COST Action “Molecular Machines”. Fraser MacMillan, United Kingdom. |
| 10:35 - 11:00 | Coffee break |
| 11:00 – 12:30 | ARBRE-MOBIEU General Assembly: Summary of Achievements during the Third Year of the Action and future perspectives. |
| 12:30 | Announcement of best poster & best talk prize & Concluding Remarks |
| 13:00 | Departure |