ARBRE:
Association of Resources for Biophysical Research in Europe
MOBIEU:
Molecular Biophysics in Europe
New Standards
Thomas Jowitt, University of Manchester, UK together with several members of the WG set up a survey on the general need of new standard systems. The survey has been filled in by members of the ARBRE/MOBIEU community and analysis was performed by Thom Jowitt. A key result is that there is indeed an unmet need for standard samples, especially for interaction studies.
- Survey on the need of new standards
- Development of a new standard system
There were several attempts to develop new standard systems for the biophysical characterization of interactions. A very promising system has been established in Thomas Jowitt’s lab, University of Manchester, UK: the binding of lysozyme to a nanobody the affinity of which can be tued by targeted amino acid exchange. After comprehensive characterization it has been used as model system in the MST benchmarking study. A general overview of this system has been presented by Thomas Jowitt at a WG meeting in 2017
New_standards_lysozyme_nanobody.PDF