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ARBRE MOBIEU Meeting 2020_
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Meeting Programme

Living Molecules: towards Integrative Biophysics of the Cell

ARBRE-MOBIEU Plenary Meeting. Prague, February 24-26, 2020.

Venue: Orea Hotel Pyramida , Prague, Czech Republic

February 24, 2020 (Monday)

9:30 – 12:30 Core group meeting (WG leaders/co-leaders only) – meeting room Jupiter & arrival (all)
9:00 – 13:00Registration – foyer
12:30 – 13:30 Lunch – hotel restaurant
13:30 – 14:00 Welcome and Introduction – Ondřej Vaněk, Patrick England – meeting room Sun II
Session 1 - Multi-level biophysics: from single-molecule to higher-order cellular assemblies
Chair: Petr Man, Czech Republic
14:00  – 14:35 Keynote lecture: Motor-independent contractility of actin rings. Zdeněk Lánský, Czech Republic .
Selected Abstracts:
14:35 – 14:55 Insights into peroxiredoxin-sulfiredoxin interaction mechanism at multiscale levels. Sophie Rahuel-Clermont, France
14:55 – 15:15N-terminus of HDAC6 is microtubule-binding domain crucial for tubulin deacetylation. Kseniya Ustinova, Czech Republic
15:15 – 15:35K27-Linked diubiquitin rapidly inhibits UCHL3 via an unusual kinetc trap. Alex Fish, The Netherlands
15:35 – 15:45Sponsor presentation: heliX® – introducing a next-generation modular biosensor for interaction and conformation analysis. Ralf Strasser (Dynamic Biosensors)
15:45 – 16:10 Coffee Break – foyer & poster area
Chair: Isabel Alves, France
16:10 – 16:20Sponsor presentation: FIDA as a new technology for characterizing protein-membrane interactions. Henrik Jensen (FIDA Technology)
Selected Abstracts:
16:20 - 16:40Modulation of cytoskeleton function by pulsed electric field: understanding from atoms to cells. Michal Cifra, Czech Republic
16:40 - 17:00Novel zinc finger-based artificial nucleases. Bálint Hajdu, Hungary
17:00 - 17:20Unravelling the mechanism of cysteine persulfide formation catalyzed by 3-mercaptopyruvate sulfurtransferases. François Talfournier, France
17:20 - 17:30Sponsor presentation: Microfluidic Diffusional Sizing (MDS) for protein characterization – latest results and next steps. Jonathan Faherty, (Fluidic Analytics)
17:30 - 17:50Flash Poster Presentations Session 1
Chair: Caroline Mas, France
1. Structure, function and interactions of NDRG1, a new possible target in lung cancer therapy induced by NI(II) and hypoxia. Ylenia Beniamino, Italy
2. Protein structure assessment combining microarray printing and infrared imaging. Joëlle De Meutter, Belgium
3. The affinity of NPM towards histones is enhanced upon their binding to DNA. Antonio Díaz-Quintana, Spain
4. FCS study of interaction of DNA and supramolecular systems functionalized with adamantyl guanidines. Tomislav Vuletić, Croatia
5. Functional and structural characterization of the encapsulin system of M. xanthus – novel cage proteins. Ana V. Almeida, Portugal
6. Importance of the disulfide link in the stability of cytosolic protein. Jasmina Rokov-Plavec, Croatia
17:50 - 18:00Sponsor presentation: Considering the concentration at which subpopulations of a heterogenous sample are characterized. Lutz Ehrhardt (Beckman Coulter)
18:00 – 19:30Poster Session 1 – meeting room Sun I
18:30 - 19:15Live demo: Mass photometry – a new way to study biomolecules. James Wilkinson (Refeyn)

February 25, 2020 (Tuesday)

Session 2 - New optical and spectroscopic tools to study events at cellular level – meeting room Sun II
Chair: Petri Kursula, Norway
9:00 - 9:35 Keynote lecture: Superresolution microscopy images: what they tell us about protein clusters – and what they don’t. Gerhard J. Schütz, Austria
Selected Abstracts:
9:35 - 9:55Understanding movement and mechanism in membrane proteins: a magnetic resonance spectroscopist’s view. Fraser MacMillan, United Kingdom
9:55 - 10:15 Investigating protein dynamics inside E. coli cells by EPR spectroscopy. Annalisa Pierro, France
10:15 - 10:35 Line-FRAP: a fast technique to measure the diffusion coefficients of fast diffusing molecules from in vitro to in vivo. Debabrata Dey, Israel
10:35 - 10:45 Sponsor presentations: Versatile tools towards real-time, single-molecule and single-cell biology. Aida Llauró-Portell (LUMICKS)
10:45 - 11:10 Coffee break – foyer & poster area
Chair: Patrick England, France
11:10 - 11:20 Sponsor presentations: Fast-track your hit screening assay development with the Buffer Exploration Kit. Amit Jean Gupta (NanoTemper)
Selected Abstracts:
11:20 - 11:40Enhanced temporal resolution in subcellular single particle tracking. Giuseppe Sancataldo, Italy
11:40 - 12:00Molecular studies using directionality of optical properties of fluorescent proteins. Josef Lazar, Czech Republic
12:00 - 12:20Benchmarking microscale thermophoresis (MST). Arthur Sedivy, Austria
12:20 - 12:40 Flash Poster Presentations Session 2:
Chair: Adriana Miele, France
1. Measuring viscoelasticity of cells by atomic force microscopy. Manfred Radmacher, Germany
2. Anomalous distribution of fluorophores in misfolded protein aggregates prepared in vitro. Claudio Canale, Italy
3. Homo- and hetero-associations drive activation of ErbB3. Timea Váradi, Hungary
4. Engineered Chimeric Carbonic Anhydrases VI and XII Switched the Structure and Affinities of Inhibitors. Joana Smirnovienė, Lithuania
5. Influence of the energetic metabolism inhibitors on the glycosylation profile of melanoma cells. Anna Sobiepanek, Poland
6. Distinct allosteric networks underlie mechanistic speciation of ABC transporters. Oded Lewinson, Israel
12:40 - 14:00Lunch – hotel restaurant
13:15 - 14:00Poster session 2 – meeting room Sun I
13:15 - 14:00Live demo: Deciphering biophysics of lipid layers, functional polymers and biomolecules by multi-parametric surface plasmon resonance. Radek Bombera (BioNavis)
Session 3 - Molecular biophysics integrative approaches – meeting room Sun II
Chair: Valeria Vetri, Italy
14:00 - 14:35 Keynote lecture: Weighing up protein assembly. Justin L. P. Benesch, United Kingdom
Selected Abstracts :
14:35 - 14:55 How do Transformer Proteins work? - Structural and thermodynamic basis of their conformational plasticity. Stefan H. Knauer, Germany
14:55 - 15:15 Biophysical investigation of the complex between the iron-responsive GATA factor Fep1 and DNA. Maria Carmela Bonaccorsi di Patti, Italy
15:15 - 15:35Pushing instruments beyond their limits: the quantitative analysis of size distributions and intrinsic viscosities using a Taylor dispersion instrument. Sébastien Brûlé, France
15:35 - 15:55Exploring the dynamic structure of bionanocages. João P. Jacinto, Portugal
15:55 - 16:05 Sponsor presentations: Easy bispecific antibody evaluation - Analyze specific binding of bsAbs in addition to IgG titer in a single high-throughput assay. Helge Schnerr (ForteBio)
16:05 -  16:30Coffee break – foyer & poster area
Chair: Petr Novák, Czech Republic
16:30  -  16:40 Sponsor presentation: Predict and evaluate stability of proteins by automated dynamic light scattering. Andrea Raffaele Abbate (Wyatt Technology)
Selected Abstracts :
16:40 -  17:00 Multivalent lectin-carbohydrates interactions studied by BLI. Jérôme Dejeu, France
17:00 -  17:20BIRDB, an infrared spectroscopy data repository. Tina Daviter, United Kingdom
17:20 -  17:40Protein-protein and protein-DNA interaction in vitro, comparing classical and innovative approaches. Paloma Fernández Varela, France
17:40 -  18:00 Flash Poster Presentations 3
Chair: Barbara Zambelli, Italy
1. Characterization of bone morphogenetic protein 2 and the Noggin antagonist. Charly Robert, Belgium
2. Antimicrobial self-assembled cyclic peptides – characterization of their membrane interactions. Bárbara Claro, Portugal
3. Determine binding affinities from thermal stability data. Stephan Niebling, Germany
4. Zinc binding sites on Tau and its self-assembly. Romain La Rocca, France
5. Biophysical characterization of cardiomyopathy-related vinculin isoforms and their interactions with actin filaments. Zeynep Aslihan Durer, Turkey
6. The first hydration level around biomolecules is site-specific. Bohdan Schneider, Czech Republic
18:00 - 19:00 Poster Session 3 – meeting room Sun I
18:00 - 19:00 Full Management Committee Meeting (members only) – meeting room Jupiter
20:00Social Dinner – walking distance, departure 19:45

February 26, 2020 (Wednesday)

Session 4 - Molecules in Action(s) – meeting room Sun II
Chair: Ondřej Vaněk, Czech Republic
9:00 – 9:20 Nuclear phosphoinositides and phase separation: nuclear lipid islets and their importance in regulation of RNA polymerase II driven transcription. Martin Sztacho, Czech Republic (CA15214 EuroCellNet - ​An integrative action for multidisciplinary studies on cellular structural structural networks)
9:20 - 9:40The advanced methods for preclinical imaging of small laboratory animals. Luděk Šefc, Czech Republic (CA17121 COMULIS - Correlated multimodal imaging in life sciences)
9:40 - 10:00 The sweet side of immunology. Luigi Lay, Italy (CA18103 INNOGLY - Innovation with glycans: new frontiers from synthesis to new biological targets)
10:00 - 10:20
Distribution of signaling molecules in membrane domains uncovered by single-molecule imaging. Alexey Bondar, Czech Republic (CA18133 ERNEST - European research network on signal transduction)
10:20 - 10:40
ELI-Beamlines: new opportunities for comprehensive biophysical studies at the advanced user facility. Anna Zymaková, Czech Republic (ELI-Beamlines - Czech centre of the Extreme Light Infrastructure ESFRI network)
10:40 - 11:10 Coffee break – foyer & poster area
11:10 – 12:40 ARBRE-MOBIEU general assembly: summary of achievements during the Action and future perspectives
12:40 – 13:00 Announcement of best poster & best talk prize. Concluding remarks
13:00Departure

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