ARBRE:
Association of Resources for Biophysical Research in Europe
MOBIEU:
Molecular Biophysics in Europe
Meeting Programme
Living Molecules: towards Integrative Biophysics of the Cell
ARBRE-MOBIEU Plenary Meeting. Prague, February 24-26, 2020.
Venue: Orea Hotel Pyramida , Prague, Czech Republic
February 24, 2020 (Monday)
| 9:30 – 12:30 | Core group meeting (WG leaders/co-leaders only) – meeting room Jupiter & arrival (all) |
| 9:00 – 13:00 | Registration – foyer |
| 12:30 – 13:30 | Lunch – hotel restaurant |
| 13:30 – 14:00 | Welcome and Introduction – Ondřej Vaněk, Patrick England – meeting room Sun II |
| Session 1 - Multi-level biophysics: from single-molecule to higher-order cellular assemblies | |
| Chair: Petr Man, Czech Republic | |
| 14:00 – 14:35 | Keynote lecture: Motor-independent contractility of actin rings. Zdeněk Lánský, Czech Republic . |
| Selected Abstracts: | |
| 14:35 – 14:55 | Insights into peroxiredoxin-sulfiredoxin interaction mechanism at multiscale levels. Sophie Rahuel-Clermont, France |
| 14:55 – 15:15 | N-terminus of HDAC6 is microtubule-binding domain crucial for tubulin deacetylation. Kseniya Ustinova, Czech Republic |
| 15:15 – 15:35 | K27-Linked diubiquitin rapidly inhibits UCHL3 via an unusual kinetc trap. Alex Fish, The Netherlands |
| 15:35 – 15:45 | Sponsor presentation: heliX® – introducing a next-generation modular biosensor for interaction and conformation analysis. Ralf Strasser (Dynamic Biosensors) |
| 15:45 – 16:10 | Coffee Break – foyer & poster area |
| Chair: Isabel Alves, France | |
| 16:10 – 16:20 | Sponsor presentation: FIDA as a new technology for characterizing protein-membrane interactions. Henrik Jensen (FIDA Technology) |
| Selected Abstracts: | |
| 16:20 - 16:40 | Modulation of cytoskeleton function by pulsed electric field: understanding from atoms to cells. Michal Cifra, Czech Republic |
| 16:40 - 17:00 | Novel zinc finger-based artificial nucleases. Bálint Hajdu, Hungary |
| 17:00 - 17:20 | Unravelling the mechanism of cysteine persulfide formation catalyzed by 3-mercaptopyruvate sulfurtransferases. François Talfournier, France |
| 17:20 - 17:30 | Sponsor presentation: Microfluidic Diffusional Sizing (MDS) for protein characterization – latest results and next steps. Jonathan Faherty, (Fluidic Analytics) |
| 17:30 - 17:50 | Flash Poster Presentations Session 1 |
| Chair: Caroline Mas, France | |
| 1. Structure, function and interactions of NDRG1, a new possible target in lung cancer therapy induced by NI(II) and hypoxia. Ylenia Beniamino, Italy | |
| 2. Protein structure assessment combining microarray printing and infrared imaging. Joëlle De Meutter, Belgium | |
| 3. The affinity of NPM towards histones is enhanced upon their binding to DNA. Antonio Díaz-Quintana, Spain | |
| 4. FCS study of interaction of DNA and supramolecular systems functionalized with adamantyl guanidines. Tomislav Vuletić, Croatia | |
| 5. Functional and structural characterization of the encapsulin system of M. xanthus – novel cage proteins. Ana V. Almeida, Portugal | |
| 6. Importance of the disulfide link in the stability of cytosolic protein. Jasmina Rokov-Plavec, Croatia | |
| 17:50 - 18:00 | Sponsor presentation: Considering the concentration at which subpopulations of a heterogenous sample are characterized. Lutz Ehrhardt (Beckman Coulter) |
| 18:00 – 19:30 | Poster Session 1 – meeting room Sun I |
| 18:30 - 19:15 | Live demo: Mass photometry – a new way to study biomolecules. James Wilkinson (Refeyn) |
February 25, 2020 (Tuesday)
| Session 2 - New optical and spectroscopic tools to study events at cellular level – meeting room Sun II |
|
| Chair: Petri Kursula, Norway | |
| 9:00 - 9:35 | Keynote lecture: Superresolution microscopy images: what they tell us about protein clusters – and what they don’t. Gerhard J. Schütz, Austria |
| Selected Abstracts: | |
| 9:35 - 9:55 | Understanding movement and mechanism in membrane proteins: a magnetic resonance spectroscopist’s view. Fraser MacMillan, United Kingdom |
| 9:55 - 10:15 | Investigating protein dynamics inside E. coli cells by EPR spectroscopy. Annalisa Pierro, France |
| 10:15 - 10:35 | Line-FRAP: a fast technique to measure the diffusion coefficients of fast diffusing molecules from in vitro to in vivo. Debabrata Dey, Israel |
| 10:35 - 10:45 | Sponsor presentations: Versatile tools towards real-time, single-molecule and single-cell biology. Aida Llauró-Portell (LUMICKS) |
| 10:45 - 11:10 | Coffee break – foyer & poster area |
| Chair: Patrick England, France | |
| 11:10 - 11:20 | Sponsor presentations: Fast-track your hit screening assay development with the Buffer Exploration Kit. Amit Jean Gupta (NanoTemper) |
| Selected Abstracts: | |
| 11:20 - 11:40 | Enhanced temporal resolution in subcellular single particle tracking. Giuseppe Sancataldo, Italy |
| 11:40 - 12:00 | Molecular studies using directionality of optical properties of fluorescent proteins. Josef Lazar, Czech Republic |
| 12:00 - 12:20 | Benchmarking microscale thermophoresis (MST). Arthur Sedivy, Austria |
| 12:20 - 12:40 | Flash Poster Presentations Session 2: |
| Chair: Adriana Miele, France | |
| 1. Measuring viscoelasticity of cells by atomic force microscopy. Manfred Radmacher, Germany | |
| 2. Anomalous distribution of fluorophores in misfolded protein aggregates prepared in vitro. Claudio Canale, Italy | |
| 3. Homo- and hetero-associations drive activation of ErbB3. Timea Váradi, Hungary | |
| 4. Engineered Chimeric Carbonic Anhydrases VI and XII Switched the Structure and Affinities of Inhibitors. Joana Smirnovienė, Lithuania | |
| 5. Influence of the energetic metabolism inhibitors on the glycosylation profile of melanoma cells. Anna Sobiepanek, Poland | |
| 6. Distinct allosteric networks underlie mechanistic speciation of ABC transporters. Oded Lewinson, Israel | |
| 12:40 - 14:00 | Lunch – hotel restaurant |
| 13:15 - 14:00 | Poster session 2 – meeting room Sun I |
| 13:15 - 14:00 | Live demo: Deciphering biophysics of lipid layers, functional polymers and biomolecules by multi-parametric surface plasmon resonance. Radek Bombera (BioNavis) |
| Session 3 - Molecular biophysics integrative approaches – meeting room Sun II | |
| Chair: Valeria Vetri, Italy | |
| 14:00 - 14:35 | Keynote lecture: Weighing up protein assembly. Justin L. P. Benesch, United Kingdom |
| Selected Abstracts : | |
| 14:35 - 14:55 | How do Transformer Proteins work? - Structural and thermodynamic basis of their conformational plasticity. Stefan H. Knauer, Germany |
| 14:55 - 15:15 | Biophysical investigation of the complex between the iron-responsive GATA factor Fep1 and DNA. Maria Carmela Bonaccorsi di Patti, Italy |
| 15:15 - 15:35 | Pushing instruments beyond their limits: the quantitative analysis of size distributions and intrinsic viscosities using a Taylor dispersion instrument. Sébastien Brûlé, France |
| 15:35 - 15:55 | Exploring the dynamic structure of bionanocages. João P. Jacinto, Portugal |
| 15:55 - 16:05 | Sponsor presentations: Easy bispecific antibody evaluation - Analyze specific binding of bsAbs in addition to IgG titer in a single high-throughput assay. Helge Schnerr (ForteBio) |
| 16:05 - 16:30 | Coffee break – foyer & poster area |
| Chair: Petr Novák, Czech Republic | |
| 16:30 - 16:40 | Sponsor presentation: Predict and evaluate stability of proteins by automated dynamic light scattering. Andrea Raffaele Abbate (Wyatt Technology) |
| Selected Abstracts : | |
| 16:40 - 17:00 | Multivalent lectin-carbohydrates interactions studied by BLI. Jérôme Dejeu, France |
| 17:00 - 17:20 | BIRDB, an infrared spectroscopy data repository. Tina Daviter, United Kingdom |
| 17:20 - 17:40 | Protein-protein and protein-DNA interaction in vitro, comparing classical and innovative approaches. Paloma Fernández Varela, France |
| 17:40 - 18:00 | Flash Poster Presentations 3 |
| Chair: Barbara Zambelli, Italy | |
| 1. Characterization of bone morphogenetic protein 2 and the Noggin antagonist. Charly Robert, Belgium | |
| 2. Antimicrobial self-assembled cyclic peptides – characterization of their membrane interactions. Bárbara Claro, Portugal | |
| 3. Determine binding affinities from thermal stability data. Stephan Niebling, Germany |
|
| 4. Zinc binding sites on Tau and its self-assembly. Romain La Rocca, France | |
| 5. Biophysical characterization of cardiomyopathy-related vinculin isoforms and their interactions with actin filaments. Zeynep Aslihan Durer, Turkey | |
| 6. The first hydration level around biomolecules is site-specific. Bohdan Schneider, Czech Republic | |
| 18:00 - 19:00 | Poster Session 3 – meeting room Sun I |
| 18:00 - 19:00 | Full Management Committee Meeting (members only) – meeting room Jupiter |
| 20:00 | Social Dinner – walking distance, departure 19:45 |
February 26, 2020 (Wednesday)
| Session 4 - Molecules in Action(s) – meeting room Sun II |
|
| Chair: Ondřej Vaněk, Czech Republic |
|
| 9:00 – 9:20 | Nuclear phosphoinositides and phase separation: nuclear lipid islets and their importance in regulation of RNA polymerase II driven transcription. Martin Sztacho, Czech Republic (CA15214 EuroCellNet - An integrative action for multidisciplinary studies on cellular structural structural networks) |
| 9:20 - 9:40 | The advanced methods for preclinical imaging of small laboratory animals. Luděk Šefc, Czech Republic (CA17121 COMULIS - Correlated multimodal imaging in life sciences) |
| 9:40 - 10:00 | The sweet side of immunology. Luigi Lay, Italy (CA18103 INNOGLY - Innovation with glycans: new frontiers from synthesis to new biological targets) |
| 10:00 - 10:20 | Distribution of signaling molecules in membrane domains uncovered by single-molecule imaging. Alexey Bondar, Czech Republic (CA18133 ERNEST - European research network on signal transduction) |
| 10:20 - 10:40 | ELI-Beamlines: new opportunities for comprehensive biophysical studies at the advanced user facility. Anna Zymaková, Czech Republic (ELI-Beamlines - Czech centre of the Extreme Light Infrastructure ESFRI network) |
| 10:40 - 11:10 | Coffee break – foyer & poster area |
| 11:10 – 12:40 | ARBRE-MOBIEU general assembly: summary of achievements during the Action and future perspectives |
| 12:40 – 13:00 | Announcement of best poster & best talk prize. Concluding remarks |
| 13:00 | Departure |
